SepQuant dropletProbe

  • Liquid surface extraction
  • Powerful alternative to Quantitative Whole Body Autoradiography
  • Maximises the use of advanced mass spectrometry platforms for analytes detection

SepQuant dropletProbe

Details

SepQuant dropletProbe Features Overview

Developed in conjunction with Oak Ridge National Laboratory, the SepQuant dropletProbe allows liquid surface extraction of analytes on surfaces and further analysis in an HPLC-MS detector.  The technique allows a powerful alternative to Quantitative Whole Body Autoradiography (QWBA) and maximises the use of advanced mass spectrometry platforms for analytes detection.

Screenshot 2017-06-04 07.50.53.png

FEATURE 1

First, the surface sample to be analyzed is mounted in a custom sample tray. Then an optical image of the mounted sample is acquired using a flatbed scanner controlled with the DropletProbe Premium© software developed at the Oak Ridge National Laboratory

FEATURE 2

Schematic of the surface sampling process including (a) determining syringe-to-surface distance, (b) the positioning of the  syringe needle 100-300 μm above a defined surface spot, (c) dispensing a discrete volume of extraction solvent onto a surface creating a liquid junction between the needle and the surface, (d) dissolution of the analyte in the extraction solvent, e) the liquid is drawn back into the syringe needle after a predefined extraction time, and (f) loaded into the sample loop followed by (g) injection for a consecutive HPLC-MS/MS analysis. Steps (c)-(e) may be repeated to improve extraction efficiency.

FEATURE 3

Extraction liquid dispensed by the needle in locked position on (a) a tissue biopsy sample and on (b) a brain thin tissue section. Zoomed in view in inset in (b) shows of liquid microjunction between the tip of the needle and the sample.

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Product Brochure

SepQuant DropletProbe – Liquid Surface Extraction Technique

SepQuant dropletProbe Features Overview

Developed in conjunction with Oak Ridge National Laboratory, the SepQuant dropletProbe allows liquid surface extraction of analytes on surfaces and further analysis in an HPLC-MS detector.  The technique allows a powerful alternative to Quantitative Whole Body Autoradiography (QWBA) and maximises the use of advanced mass spectrometry platforms for analytes detection.

Screenshot 2017-06-04 07.50.53.png

FEATURE 1

First, the surface sample to be analyzed is mounted in a custom sample tray. Then an optical image of the mounted sample is acquired using a flatbed scanner controlled with the DropletProbe Premium© software developed at the Oak Ridge National Laboratory

FEATURE 2

Schematic of the surface sampling process including (a) determining syringe-to-surface distance, (b) the positioning of the  syringe needle 100-300 μm above a defined surface spot, (c) dispensing a discrete volume of extraction solvent onto a surface creating a liquid junction between the needle and the surface, (d) dissolution of the analyte in the extraction solvent, e) the liquid is drawn back into the syringe needle after a predefined extraction time, and (f) loaded into the sample loop followed by (g) injection for a consecutive HPLC-MS/MS analysis. Steps (c)-(e) may be repeated to improve extraction efficiency.

FEATURE 3

Extraction liquid dispensed by the needle in locked position on (a) a tissue biopsy sample and on (b) a brain thin tissue section. Zoomed in view in inset in (b) shows of liquid microjunction between the tip of the needle and the sample.

Product Brochure

SepQuant DropletProbe - Liquid Surface Extraction Technique

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