UHPLC with Electrochemical Detection

  • Optimised in every detail
  • Designed for performance
  • Parallel analysis with multiple flow cells
  • Micro volume sample handling



Ultra High Performance Liquid Chromatography (UHPLC) has gained much interest in many application areas. Small particle (sub 2 um) stationary phases with high separation efficiency makes it possible to use shorter columns and a fast analysis time while maintaining excellent separation. The DECADE Elite has been developed to meet the demands in fast analysis, as to detection sensitivity, acquisition frequency and temperature of separation.

The DECADE Elite uses Antec’s workstation concept, keeping the column and flow cell (separation and detection) at a very accurate and stable temperature. Stable working conditions are a prerequisite for trace analysis, without stability no sensitivity! In addition, the DECADE Elite has ADF (advanced digital filter) which is a noise elimination algorithm specially developed for electrochemical data. It further improves the detection limit, depending on conditions up to a factor 200. Detection sensitivity is especially eminent in neurotransmitter analysis. Together with the new SenCell bottom line detection limits down to 30 pmol/L have been obtained in 2 µL sample. Antec provides a number of amperometric flow cells with different features for specific applications. The flow cells are certified and warranted up to 5 years (glassy carbon type).

The DECADE Elite is able to control up to 4 cells for high throughput applications or electrochemical pre-reduction (e.g. vit-K, Q10 etc). The detector has a pulse mode for carbohydrate analysis using the traditional 3-step pulse and more advanced 4 or 5 step pulses. The scan mode is available for method optimization, to characterize the electrochemical behaviour of a substance of interest.

The ALEXYS™ is a HPLC system with electrochemical detection that has been designed to meet the highest demands for detection sensitivity and performance. The ALEXYS is easily upgradeable to micro-bore and capillary LC-EC. The system uses Clarity, a fully featured data acquisition software with all the tools for automated data acquisition, calibration, processing and reporting.

An ALEXYS analyzer additionally has the appropriate kit that is optimized for a specific application. For example, guaranteed applications have been developed for the analysis of neurotransmitters such as noradrenaline, dopamine, serotonin and metabolites in microdialysates. Typically, picomolar concentration detection limits have been obtained in only a few microliters of sample. Parallel detection schemes using multiple flow cells have been developed for time, sample and experimental cost savings! The ALEXYS has proven its performance in routine analysis in clinical, food & beverage, environmental and pharmaceutical laboratories as well.

Due to its incredible sensitivity, EC detection has very high demands from an LC system. To fully exploit LC-ECD, Antec Leyden has created a system that meets these demands. This dedicated system is comprised of the DECADE II, autosampler, pump and data acquisition software. An organizer rack with a high-efficiency integrated degasser, up to 2 pulse dampeners and a mains
power distributor for all devices are also part of the system. The ALEXYS™ is easily upgradeable to allow micro-bore and capillary LC-ECD. The fully featured data acquisition software contains all the tools for automated sampling, data acquisition, calibration, processing and reporting.

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