SepQuant dropletProbe Features Overview

Developed in conjunction with Oak Ridge National Laboratory, the SepQuant dropletProbe allows liquid surface extraction of analytes on surfaces and further analysis in an HPLC-MS detector.  The technique allows a powerful alternative to Quantitative Whole Body Autoradiography (QWBA) and maximises the use of advanced mass spectrometry platforms for analytes detection.

FEATURE 1

First, the surface sample to be analyzed is mounted in a custom sample tray. Then an optical image of the mounted sample is acquired using a flatbed scanner controlled with the DropletProbe Premium© software developed at the Oak Ridge National Laboratory

FEATURE 2

Schematic of the surface sampling process including (a) determining syringe-to-surface distance, (b) the positioning of the  syringe needle 100-300 μm above a defined surface spot, (c) dispensing a discrete volume of extraction solvent onto a surface creating a liquid junction between the needle and the surface, (d) dissolution of the analyte in the extraction solvent, e) the liquid is drawn back into the syringe needle after a predefined extraction time, and (f) loaded into the sample loop followed by (g) injection for a consecutive HPLC-MS/MS analysis. Steps (c)-(e) may be repeated to improve extraction efficiency.

FEATURE 3

Extraction liquid dispensed by the needle in locked position on (a) a tissue biopsy sample and on (b) a brain thin tissue section. Zoomed in view in inset in (b) shows of liquid microjunction between the tip of the needle and the sample.

Product Brochure

SepQuant DropletProbe – Liquid Surface Extraction Technique

TM Sprayer Application Notes

• Forensic Science
• Environmental Science
• Proteomics Discovery
• Drug Development